Product Description

Desmoglein-3 (DSG3) is a calcium-binding transmembrane glycoprotein belonging to the cadherin superfamily. It is an important structural component of desmosomes, specialized intercellular junctions that provide mechanical strength and adhesion between neighboring epithelial cells.

This monoclonal antibody recognizes a protein of approximately 130 kDa identified as Desmoglein-3. It exhibits high specificity for DSG3 and does not cross-react with other members of the desmoglein family, according to the supplied product information.

DSG3 is widely investigated as a tissue marker in squamous cell carcinoma (SqCC), particularly in lung cancer research. Its expression can help distinguish squamous cell carcinoma from other histological subtypes when evaluated alongside appropriate morphological and immunohistochemical markers.

This antibody is conjugated to CF405S, a blue fluorescent dye designed for fluorescence-based detection. CF® fluorescent dyes offer brightness and photostability for labeled antibody applications.

Key Features & Benefits

Biological Role of Desmoglein-3

Desmosomes are specialized cell junctions that connect adjacent epithelial cells and contribute to tissue integrity. Desmoglein-3 is a transmembrane adhesion protein that participates in these structures by mediating calcium-dependent cell-cell interactions.

Alterations in DSG3 expression have been investigated in several epithelial cancers. In lung pathology research, DSG3 is particularly relevant because its expression is commonly associated with squamous differentiation.

Studying DSG3 can therefore contribute to understanding epithelial tissue organization, cellular adhesion and molecular characteristics associated with squamous cell carcinoma.

Research Applications

• Squamous Cell Carcinoma Research: Investigation of DSG3 expression in squamous cell carcinoma models and tissue samples.
• Lung Cancer Research: Evaluation of squamous differentiation and comparison with other lung cancer subtypes.
• Fluorescence Microscopy: Detection of DSG3 using compatible fluorescence imaging systems.
• Cell Adhesion Studies: Investigation of desmosomal proteins and epithelial cell-cell interactions.
• Immunological Research: Study of protein expression and tissue-specific molecular markers.
• Protein Localization: Examination of Desmoglein-3 distribution in epithelial structures.

Technical Specifications

Fluorescence Detection Considerations

• Use fluorescence instrumentation compatible with the CF405S fluorophore.
• Consider the excitation and emission characteristics of CF405S when selecting imaging settings.
• Blue fluorescent conjugates may produce lower signal intensity than other dye colors.
• For low-abundance targets, evaluate signal-to-background performance before selecting the detection configuration.
• Use appropriate negative and positive controls to assess antibody specificity and staining quality.

Storage & Handling

• Follow the manufacturer's recommended storage conditions.
• Protect fluorescently labeled antibodies from unnecessary light exposure.
• Avoid repeated freeze-thaw cycles where possible.
• Use validated experimental protocols and appropriate laboratory handling procedures.